View example sentences and word forms for Avidin.
Avidin
Avidin meaning
A tetrameric protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs.
Example sentences (4)
Avidin denatures upon heating (cooking).
Because both streptavidin and avidin bind biotin with high affinity (K d of 10 −14 mol/l to 10 −15 mol/l) and specificity, biotinylated proteins of interest can be isolated from a sample by exploiting this highly stable interaction.
The sample is incubated with streptavidin/avidin beads, allowing capture of the biotinylated protein of interest.
To circumvent this problem, beads conjugated to monomeric avidin can be used, which has a decreased biotin-binding affinity of ~10 −8 mol/l, allowing the biotinylated protein of interest to be eluted with excess free biotin.