View example sentences and word forms for Ligase.
Ligase meaning
Any of a group of enzymes that catalyze the binding of two molecules; synthetase.
Example sentences (11)
Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, citation with DNA ligase creating the final phosphodiester bond to fully repair the DNA.
The molecular alarm system for mitochondrial quality control involves two enzymes: the protein kinase PINK1, which chemically modifies proteins with phosphate, and the ubiquitin ligase PARKIN, which marks with a molecule called ubiquitin.
DNA ligase is used in both DNA repair and DNA replication (see Mammalian ligases ).
HIF is synthesized consititutively, and hydroxylation of at least one of two critical proline residues mediates their interaction with the von Hippel Lindau E3 ubiquitin ligase complex, which targets them for rapid degradation.
It can also ligate blunt-ended DNA with much greater efficiency than E. coli DNA ligase.
It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV ) may specifically repair double-strand breaks (i.e. a break in both complementary strands of DNA).
Ligase can join two complementary fragments of nucleic acid and repair single stranded breaks that arise in double stranded DNA during replication.
Ligase works to fill these nicks in, thus completing the newly replicated DNA molecule.
The absence of stably annealed ends also means that the ligation efficiency is lowered, requiring a higher ligase concentration to be used.
This step cuts out the DNA transposon, which is then ligated into a new target site; the process involves activity of a DNA polymerase that fills in gaps and of a DNA ligase that closes the sugar-phosphate backbone.
Topoisomerases and helicases Topoisomerases are enzymes with both nuclease and ligase activity.