Microsatellite in a sentence

The oligonucleotide probe hybridizes with the repeat in the microsatellite, and the probe/microsatellite complex is then pulled out of solution.

An earlier version of this article incorrectly referred to Tetra-1 as a microsatellite.

The Prox-1 microsatellite developed by students at Georgia Tech deployed 230-foot-long Terminator Tape last year.

Among the microsatellite sequences, trinucleotide repeats are of particular importance, as sometimes occur within coding regions of genes for proteins and may lead to genetic disorders.

Another possible cause of microsatellite mutations are point mutations, where only one nucleotide is incorrectly copied during replication.

A number of DNA samples from specimens of Littorina plena amplified using polymerase chain reaction with primers targeting a variable simple sequence repeat (SSR, a.k.a. microsatellite) locus.

By examining 10 microsatellite loci, researchers found two genetically distinct subpopulations of coconut—one originating in the Indian Ocean, the other in the Pacific Ocean.

Colonies are then developed, and screened with fluorescently–labelled oligonucleotide sequences that will hybridize to a microsatellite repeat, if present on the DNA segment.

DNA is repeatedly denatured at a high temperature to separate the double strand, then cooled to allow annealing of primers and the extension of nucleotide sequences through the microsatellite.

Forensic analysis A partial human STR profile obtained using the Applied Biosystems Identifiler kit Microsatellite analysis became popular in the field of forensics in the 1990s.

Increased heterozygosity in a population will also increase microsatellite mutation rates, citation especially when there is a large length difference between alleles.

Once the potentially useful microsatellites are determined, the flanking sequences can be used to design oligonucleotide primers which will amplify the specific microsatellite repeat in a PCR reaction.

Others are located in regulatory or even coding DNA - microsatellite mutations in such cases can lead to phenotypic changes and diseases.

Random microsatellite primers can be developed by cloning random segments of DNA from the focal species.

Repeated sequences of fewer than ten nucleotides (e.g. the dinucleotide repeat (AC) n ) are termed microsatellite sequences.

Structures and locations A microsatellite consists of a tract of tandemly repeated (i.e. adjacent) DNA motifs that range in length from two to five nucleotides, and are typically repeated 5-50 times.

Their goal is to accelerate a one-kilogram microsatellite into low Earth orbit using a custom-built, one megawatt ground-based laser.

This is likely due to homologous chromosomes with arms of unequal lengths causing instability during meiosis. citation Direct estimates of microsatellite mutation rates have been made in numerous organisms, from insects to humans.

This process involves significant trial and error on the part of researchers, as microsatellite repeat sequences must be predicted and primers that are randomly isolated may not display significant polymorphism.

Thus, the mutation rate at microsatellite loci is expected to differ from other mutation rates, such as base substitution rates.